Synthesis, Characterization, and Cytotoxicity Evaluation of Chlorambucil-Functionalized Mesoporous Silica Nanoparticles.
Juliana Camila Fischer KarnoppJuliana JorgeJaqueline Rodrigues da SilvaDiego BoldoKristiane Fanti Del Pino SantosAdriana Pereira DuarteGustavo Rocha de CastroRicardo Bentes de AzevedoAriadna Lafourcade PradaJesus Rafael Rodriguez AmadoMarco Antonio Utrera MartinesPublished in: Pharmaceutics (2024)
This study describes the synthesis and characterization of chlorambucil (CLB)-functionalized mesoporous silica nanoparticles (MSNs) for potential application in cancer therapy. The nanoparticles were designed with a diameter between 20 and 50 nm to optimize cellular uptake and avoid rapid clearance from the bloodstream. The synthesis method involved modifying a previously reported technique to reduce particle size. Successful functionalization with CLB was confirmed through various techniques, including Fourier transform infrared spectroscopy (FTIR) and elemental analysis. The cytotoxicity of the CLB-functionalized nanoparticles (MSN@NH 2 -CLB) was evaluated against human lung adenocarcinoma cells (A549) and colon carcinoma cells (CT26WT). The results suggest significantly higher cytotoxicity of MSN@NH 2 -CLB compared to unbound CLB, with improved selectivity towards cancer cells over normal cells. This suggests that MSN@NH 2 -CLB holds promise as a drug delivery system for targeted cancer therapy.
Keyphrases
- cancer therapy
- induced apoptosis
- drug delivery
- cell cycle arrest
- quantum dots
- room temperature
- endothelial cells
- computed tomography
- endoplasmic reticulum stress
- molecularly imprinted
- walled carbon nanotubes
- oxidative stress
- escherichia coli
- signaling pathway
- contrast enhanced
- machine learning
- perovskite solar cells
- klebsiella pneumoniae
- risk assessment
- solid phase extraction
- optic nerve