Hematopoietic differentiation persists in human iPSCs defective in de novo DNA methylation.

Olivia CyprisJulia FranzenJoana FrobelPhilipp GlückChao-Chung KuoStephani SchmitzSelina NüchternMartin ZenkeWolfgang Wagner

Published in: BMC biology (2022)

iHPCs even gained growth advantage-despite loss of almost the entire de novo DNA methylation. Partial recapitulation of DNA methylation patterns of AML with DNMT3A mutations by our DNMT3A knockout iHPCs indicates that our model system can help to elucidate mechanisms of clonal hematopoiesis.

Keyphrases

dna methylation
genome wide
gene expression
endothelial cells
induced pluripotent stem cells
acute myeloid leukemia
copy number
bone marrow
allogeneic hematopoietic stem cell transplantation