Anti-cancer & anti-metastasis properties of bioorganic-capped silver nanoparticles fabricated from Juniperus chinensis extract against lung cancer cells.
Hassan NoorbazarganSobhan AmintehraniAghigh DolatabadiAinaz MashayekhiNazanin KhayamPooria MoulaviMohammad NaghizadehAmir MirzaieFatmeh Mirzaei RadMahsa KavousiPublished in: AMB Express (2021)
The current study evaluated the anti-cancer properties of bio-functionalized silver nanoparticles fabricated by Juniperus chinensis leaf extracts. The nanoparticles were characterized by scanning electron microscopy, transmission electron microscopy, UV-visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, dynamic light scattering, Zeta potential and X-ray spectroscopy. Further, this study elucidated the cellular and molecular mechanisms of nanoparticles for anti-proliferative and apoptotic effects on human lung cancer cells (A549) and compared them with commercial drug cisplatin. The size of the spherical nanoparticle was 12.96 nm with negative zeta potential. Up-regulation of caspase 3,9 and p53, Annexin V-FITC/PI, DAPI staining, and ROS production indicated the remarkable apoptotic effect of AgNPs compared to cisplatin. Moreover, down-regulation of MMP2/MMP9 scratch and matrigel assays revealed anti-metastatic properties of AgNPs. Cell cycle analysis and downregulation of cyclin D1 indicated cancer cell cessation in the G0/G1 phase. Overall, the results revealed that the green-synthetized AgNPs had anti-metastasis and anti-proliferation effects on lung cancer cells in comparison to cisplatin with lower side effects on the normal cell line.
Keyphrases
- silver nanoparticles
- electron microscopy
- cell cycle
- cell death
- high resolution
- cell proliferation
- squamous cell carcinoma
- single molecule
- signaling pathway
- endothelial cells
- emergency department
- single cell
- oxidative stress
- magnetic resonance imaging
- photodynamic therapy
- climate change
- contrast enhanced
- solid phase extraction
- dual energy