Compact, Polyvalent Mannose Quantum Dots as Sensitive, Ratiometric FRET Probes for Multivalent Protein-Ligand Interactions.
Yuan GuoChadamas SakonsinsiriInga NehlmeierMartin A FascioneHaiyan ZhangWeili WangStefan PöhlmannW Bruce TurnbullDejian ZhouPublished in: Angewandte Chemie (Weinheim an der Bergstrasse, Germany) (2016)
A highly efficient cap-exchange approach for preparing compact, dense polyvalent mannose-capped quantum dots (QDs) has been developed. The resulting QDs have been successfully used to probe multivalent interactions of HIV/Ebola receptors DC-SIGN and DC-SIGNR (collectively termed as DC-SIGN/R) using a sensitive, ratiometric Förster resonance energy transfer (FRET) assay. The QD probes specifically bind DC-SIGN, but not its closely related receptor DC-SIGNR, which is further confirmed by its specific blocking of DC-SIGN engagement with the Ebola virus glycoprotein. Tuning the QD surface mannose valency reveals that DC-SIGN binds more efficiently to densely packed mannosides. A FRET-based thermodynamic study reveals that the binding is enthalpy-driven. This work establishes QD FRET as a rapid, sensitive technique for probing structure and thermodynamics of multivalent protein-ligand interactions.
Keyphrases
- energy transfer
- quantum dots
- dendritic cells
- living cells
- highly efficient
- sensitive detection
- single molecule
- fluorescent probe
- hepatitis c virus
- binding protein
- nitric oxide
- human immunodeficiency virus
- hiv positive
- social media
- transcription factor
- hiv aids
- single cell
- photodynamic therapy
- amino acid
- hiv testing
- dna binding