Dendrocrepidamine, a novel octahydroindolizine alkaloid from the roots of Dendrobium crepidatum.
Xiao-Qian DingYu-Qing ZouJian LiuXia-Chang WangYang HuXiao LiuChao-Feng ZhangPublished in: Journal of Asian natural products research (2021)
A novel octahydroindolizine alkaloid, named dendrocrepidamine (1) with an unusual 18,19,19'-cyclopropanone-dendrocrepine skeleton, was isolated from the ethanol extract of the roots of Dendrobium crepidatum, along with six known compounds (2-7). The structure of 1 was elucidated through HR-ESIMS, NMR spectroscopic data and computational calculations. All compounds were examined for their inhibitory effects on nitric oxide (NO) production induced by lipopolysaccharide (LPS) in RAW264.7 cells with IC50 values in the range of 3.04-54.89 µM. In vivo, crepidatin (6) (80, 40 and 10 mg/kg) showed a significant protective effect against LPS-induced acute lung injury (ALI) in mice.
Keyphrases
- lps induced
- inflammatory response
- nitric oxide
- lipopolysaccharide induced
- induced apoptosis
- toll like receptor
- cell cycle arrest
- anti inflammatory
- oxidative stress
- magnetic resonance
- molecular docking
- electronic health record
- high resolution
- molecular dynamics
- molecular dynamics simulations
- density functional theory
- high fat diet induced
- big data
- hydrogen peroxide
- adipose tissue
- immune response
- type diabetes
- artificial intelligence
- machine learning
- wild type
- insulin resistance
- cell proliferation
- mass spectrometry