Repeatability and reproducibility of the Forskolin-induced swelling (FIS) assay on intestinal organoids from people with Cystic Fibrosis.

Marlou C BierlaaghAnabela S RamalhoIris A L SilvaAnnelotte M VonkRutger M van den BorPeter van MourikJohanna PottSylvia W F SuenSylvia F BojRobert G J VriesElise LammertynFrançois VermeulenMargarida D AmaralKris de BoeckCornelis K van der EntJeffrey M Beekman

Published in: Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society (2024)

The observed repeatability and reproducibility of the FIS assay within and across different labs is high and support the use of FIS as biomarker of CFTR function in the presence or absence of CFTR modulators.

Keyphrases

cystic fibrosis
high throughput
high glucose
small molecule
diabetic rats
drug induced
oxidative stress