Validation of the Thermo Scientific™ SureTect™ Escherichia coli O157: H7 and STEC Screening PCR Assay and SureTect™ Escherichia coli STEC Identification PCR Assay for the Detection of Escherichia coli O157: H7 and the Escherichia coli STEC Serotypes (O26, O45, O103, O111, O121, O145) from Fresh Raw Spinach, Fresh Baby Leaves, Fresh Cut Tomatoes, Frozen Raw Beef, Raw Beef Trim and Beef Carcass Sponges AOAC Performance Tested MethodSM 012102.
Katharine EvansNikki FauldsJessica WilliamsAna-Maria LeonteDavid CrabtreeKatherine ChurchDean LeakDaniele SohierJukka-Pekka PalomäkiPauliina HeikkinenCraig MantheKateland M KochBenjamin BastinM Joseph BenzingerJames AginPublished in: Journal of AOAC International (2021)
Results are obtained in 80 minutes post-enrichment with both assays run simultaneously, allowing for the detection and confirmation of STEC within a single workflow.