A Single Aptamer-Dependent Sandwich-Type Biosensor for the Colorimetric Detection of Cancer Cells via Direct Coordinately Binding of Bare Bimetallic Metal-Organic Framework-Based Nanozymes.
Yuhui ZhuXueting FangXiaofei LvMeijun LuHui XuShengqiang HuShulin ZhaoFanggui YePublished in: Biosensors (2023)
A typical colorimetric sandwich-type sensor relies on dual antibodies/aptamers to specifically visualize the targets. The requirement of dual antibodies/aptamers and low signal intensity inevitably increases the design difficulty and compromises the sensing sensitivity. In this work, a novel sandwich-type aptasensor was developed using single aptamer-functionalized magnetic nanoparticles as a specific recognition unit to target cancer cells and a bimetallic metal-organic frameworks (MOFs)-based nanozymes as a colorimetric signal amplification unit. The well-defined crystalline structure of UIO-66 MOFs enabled the introduction of Fe/Zr bimetal nodes, which possessed integrated properties of the peroxidase-like nanozyme activity and direct coordinately binding to the cell surface. Such a novel construction strategy of sandwich-type aptasensors achieved simple, sensitive, and specific detection of the target cancer cells, which will inspire the development of biosensors.
Keyphrases
- metal organic framework
- label free
- gold nanoparticles
- sensitive detection
- hydrogen peroxide
- loop mediated isothermal amplification
- quantum dots
- cell surface
- fluorescent probe
- nucleic acid
- computed tomography
- squamous cell carcinoma
- rectal cancer
- early stage
- mass spectrometry
- real time pcr
- binding protein
- dna binding