Investigation of the mixed origins of the MGC-803 cell line reveals that it is a hybrid cell line derived from HeLa.
Meimei YangJing HeSixuan XiaYudong WangJun XiongCong LiaoNan LiSanfu QuChao ShenPublished in: Human cell (2023)
Human cancer cell lines have an essential role in cancer research, but only authentic cell lines should be used as biological models. Authentication testing using short tandem repeat (STR) loci has shown that MGC-803 cells, which were reported to come from gastric adenocarcinoma, are similar to HeLa. In this study, we confirmed that the MGC-803 cell line contains genetic material from HeLa, including genetic sequence from human papilloma virus 18 (HPV18). Additional alleles were present on STR analysis that remained stable after extensive passaging and generation of mono-clones. This behavior is consistent with a hybrid cell line arising from cell-cell fusion. Further genetic analysis revealed that MGC-803 originated from donors with different genetic ancestries, one African (HeLa) and the other Asian. Transcriptomic analysis demonstrated that MGC-803 closely resembles HeLa and another nasopharyngeal-HeLa hybrid cell line CNE-2. Based on these findings, we conclude that MGC-803 is a hybrid cell line derived from HeLa and other cells, the latter derived from a different patient with Asian genetic ancestry.
Keyphrases
- cell cycle arrest
- cell death
- pi k akt
- genome wide
- endothelial cells
- single cell
- induced apoptosis
- papillary thyroid
- copy number
- cell therapy
- squamous cell carcinoma
- signaling pathway
- dna methylation
- stem cells
- cell proliferation
- young adults
- gene expression
- radiation therapy
- endoplasmic reticulum stress
- genome wide association study
- kidney transplantation
- mesenchymal stem cells