A capillary-based multiplexed isothermal nucleic acid-based test for sexually transmitted diseases in patients.
Gaolian XuHang ZhaoJonathan M CooperJulien ReboudPublished in: Chemical communications (Cambridge, England) (2018)
We demonstrate a multiplexed loop mediated isothermal amplification (LAMP) assay for infectious disease diagnostics, where the analytical process flow of target pathogens genomic DNA is performed manually by moving magnetic beads through a series of plugs in a capillary. Heat is provided by a water bath and the results are read by the naked eye, enabling applications in low resource settings.
Keyphrases
- nucleic acid
- loop mediated isothermal amplification
- end stage renal disease
- infectious diseases
- newly diagnosed
- ejection fraction
- chronic kidney disease
- single molecule
- prognostic factors
- peritoneal dialysis
- high throughput
- sensitive detection
- molecularly imprinted
- gene expression
- dna methylation
- antimicrobial resistance
- solid phase extraction