Antibacterial Synthetic Nanocelluloses Synergizing with a Metal-Chelating Agent.
Takeshi SerizawaSaeko YamaguchiKai SugiuraRamona MartenAkihisa YamamotoYuuki HataToshiki SawadaHiroshi TanakaMotomu TanakaPublished in: ACS applied bio materials (2023)
Antibacterial materials composed of biodegradable and biocompatible constituents that are produced via eco-friendly synthetic strategies will become an attractive alternative to antibiotics to combat antibiotic-resistant bacteria. In this study, we demonstrated the antibacterial properties of nanosheet-shaped crystalline assemblies of enzymatically synthesized aminated cellulose oligomers (namely, surface-aminated synthetic nanocelluloses) and their synergy with a metal-chelating antibacterial agent, ethylenediaminetetraacetic acid (EDTA). Growth curves and colony counting assays revealed that the surface-aminated cellulose assemblies had an antibacterial effect against Gram-negative Escherichia coli ( E. coli ). The cationic assemblies appeared to destabilize the cell wall of E. coli through electrostatic interactions with anionic lipopolysaccharide (LPS) molecules on the outer membrane. The antibacterial properties were significantly enhanced by the concurrent use of EDTA, which potentially removed metal ions from LPS molecules, resulting in synergistic bactericidal effects. No antibacterial activity of the surface-aminated cellulose assemblies was observed against Gram-positive Staphylococcus aureus even in the presence of EDTA, further supporting the contribution of electrostatic interactions between the cationic assemblies and anionic LPS to the activity against Gram-negative bacteria. Analysis using quartz crystal microbalance with dissipation monitoring revealed the attractive interaction of the surface-aminated cellulose assembly with LPS Ra monolayers artificially produced on the device substrate.
Keyphrases
- silver nanoparticles
- anti inflammatory
- gram negative
- escherichia coli
- inflammatory response
- ionic liquid
- multidrug resistant
- staphylococcus aureus
- essential oil
- cell wall
- aqueous solution
- drug delivery
- radiation therapy
- single cell
- squamous cell carcinoma
- toll like receptor
- high throughput
- molecular dynamics simulations
- biofilm formation
- quantum dots
- locally advanced
- immune response
- pseudomonas aeruginosa
- candida albicans
- ankylosing spondylitis
- drug release
- interstitial lung disease