A Far-Red Emitting Fluorescent Chemogenetic Reporter for In Vivo Molecular Imaging.
Chenge LiAlison G TeboMarion ThauvinMarie-Aude PlamontMichel VolovitchXavier MorinSophie VrizArnaud GautierPublished in: Angewandte Chemie (International ed. in English) (2020)
Far-red emitting fluorescent labels are highly desirable for spectral multiplexing and deep tissue imaging. Here, we describe the generation of frFAST (far-red Fluorescence Activating and absorption Shifting Tag), a 14-kDa monomeric protein that forms a bright far-red fluorescent assembly with (4-hydroxy-3-methoxy-phenyl)allylidene rhodanine (HPAR-3OM). As HPAR-3OM is essentially non-fluorescent in solution and in cells, frFAST can be imaged with high contrast in presence of free HPAR-3OM, which allowed the rapid and efficient imaging of frFAST fusions in live cells, zebrafish embryo/larvae, and chicken embryos. Beyond enabling the genetic encoding of far-red fluorescence, frFAST allowed the design of a far-red chemogenetic reporter of protein-protein interactions, demonstrating its great potential for the design of innovative far-red emitting biosensors.
Keyphrases
- quantum dots
- living cells
- induced apoptosis
- energy transfer
- fluorescent probe
- high resolution
- crispr cas
- cell cycle arrest
- gene expression
- signaling pathway
- label free
- magnetic resonance imaging
- dna methylation
- oxidative stress
- sensitive detection
- mass spectrometry
- endoplasmic reticulum stress
- optical coherence tomography
- pi k akt
- heat shock protein
- binding protein