Login / Signup

Novel Application of Photo-Crosslinked Urocanic-Acid-Modified Chitosan in Corneal Wounds.

An-Li LeeHao-Ying HsiehYun-Yu ChenLi-Hui TsaiShiuan-Li WeyDai-Shi ChenYi-Jane ChenTai-Horng Young
Published in: ACS biomaterials science & engineering (2022)
In the last few years, the use of tissue adhesives in corneal perforation has gained immense popularity in clinical practices. The present study aimed to devise a new application of urocanic-acid-modified chitosan (CS) with methylene blue (MB) as a photosensitizer for the development of a photo-crosslinked tissue adhesive. In particular, the curing time was controlled with the aid of a 650 nm red diode. Under the same irradiation condition, the mechanical properties were tuned using the photosensitizer at different concentrations. In vitro tests revealed that the gel was ductile and biocompatible. The application of the gel to a perforated cornea model stopped the leakage of aqueous humor, immediately after the gel was photo-crosslinked. The blue appearance of the gel provided high precision when applied to corneal wounds. Importantly, the crosslinked gel became transparent within 24 h, owing to the dissipation of MB from tears, and the gel spontaneously sloughed off without artificial removal. Altogether, the study reported the development of a novel photo-crosslinkable urocanic-acid-modified CS gel that exhibited significant potential to be utilized in the healing of corneal perforation.
Keyphrases
  • wound healing
  • hyaluronic acid
  • photodynamic therapy
  • optical coherence tomography
  • healthcare
  • drug delivery
  • single cell
  • radiation therapy
  • risk assessment
  • climate change