Multiplex Photoluminescent Silicon Nanoprobe for Diagnostic Bioimaging and Intracellular Analysis.

Herein, a label-free multiplex photoluminescent silicon nanoprobe (PLSN-probe) is introduced as a potential substitute for quantum dots (QDs) in bioimaging. An inherently non-photoluminescent silicon substrate is altered to create the PLSN-probe, to overcome the major drawbacks of presently available QDs. Additionally, crystallinity alterations of the multiplane crystalline PLSN-probes lead to broad absorption and multiplex fluorescence emissions, which are attributed to the simultaneous existence of multiple crystal planes. The PLSN-probe not only demonstrates unique optical properties that can be exploited for bioimaging but also exhibits cell-selective uptake that allows the differentiation and diagnosis of HeLa and fibroblast cells. Moreover, multiplex emissions of the PLSN-probe illuminate different organelles such as the nucleus, nucleolemma, and cytoskeleton, depending on size-based preferential uptake by the cell organs. This in vitro study reveals that cancerous HeLa cells have a higher propensity for taking up the PLSN-probe compared to fibroblast cells, allowing the diagnosis of cancerous HeLa cells. Additionally, the fluorescence intensity per unit area of the cell is found to be a reliable means for distinguishing between dead and healthy cells. It is anticipated that the multifunctionality of the PLSN-probes will lead to better insight into the use of such probes for bioimaging and diagnosis applications.