A Simple GC-MS/MS Method for Determination of Smoke Taint-Related Volatile Phenols in Grapes.
Zhiqian LiuVilnis EzernieksPriyanka ReddyAaron ElkinsChristian KrillKieran MurphySimone J RochfortGerman SpangenbergPublished in: Metabolites (2020)
Volatile phenols (VPs) derived from smoke-exposed grapes are known to confer a smoky flavor to wine. Current methods for determination of VPs in grape berries either involve complex sample purification/derivatization steps or employ two analytical platforms for free and bound VP fractions. We report here a simple gas chromatography-tandem mass spectrometry (GC-MS/MS) method for quantification of both free and bound VPs in grapes, based on optimized (1) GC-MS/MS parameters, (2) an analyte extraction procedure, and (3) phenol glycoside hydrolysis conditions. Requiring neither sample cleanup nor a derivatization step, this method is sensitive (LOD ≤ 1 ng/g berries) and reproducible (RSD < 12% for repeated analyses) and is expected to significantly reduce the sample turnover time for smoke taint detection in vineyards.
Keyphrases
- gas chromatography
- solid phase extraction
- tandem mass spectrometry
- ultra high performance liquid chromatography
- high performance liquid chromatography
- liquid chromatography
- liquid chromatography tandem mass spectrometry
- gas chromatography mass spectrometry
- molecularly imprinted
- simultaneous determination
- high resolution mass spectrometry
- mass spectrometry
- ms ms
- high resolution
- minimally invasive
- bone mineral density
- loop mediated isothermal amplification
- postmenopausal women
- body composition