Development of a Novel Multiplex PCR Method for the Rapid Detection of SARS-CoV-2, Influenza A Virus, and Influenza B Virus.

Liang MaHaoyan ZhuYongwei Jiang Xiaomu Kong Peng Gao Yi Liu Meimei ZhaoGuoxiong DengYongtong Cao

Published in: International journal of analytical chemistry (2024)

A multiplex real-time quantitative fluorescence RT-PCR system for three respiratory viruses of SARS-CoV-2, FluA, and FluB was established with a high amplification efficiency and sensitivity reaching 200 copies/mL for all samples. Combined with the automated microfluidic nucleic acid detection system, the system can achieve rapid detection in 30 minutes.

Keyphrases

real time pcr
sars cov
nucleic acid
high throughput
loop mediated isothermal amplification
respiratory syndrome coronavirus
label free
machine learning
deep learning
single molecule
mass spectrometry
energy transfer
respiratory tract