PAT-H-MS coupled with laser microdissection to study histone post-translational modifications in selected cell populations from pathology samples.

Roberta NoberiniRémi LonguespéeCristina RichichiGiancarlo PruneriMark KriegsmannGiuliana PelicciTiziana Bonaldi

Published in: Clinical epigenetics (2017)

These results show the feasibility of applying techniques to reduce sample heterogeneity, including laser microdissection, to the PAT-H-MS protocol, providing new tools in clinical epigenetics and opening new avenues for the comprehensive analysis of histone post-translational modifications in selected cell populations.

Keyphrases

single cell
mass spectrometry
multiple sclerosis
cell therapy
ms ms
dna methylation
stem cells
gene expression
high speed
mesenchymal stem cells
genetic diversity