Monkey dorsolateral prefrontal cortex shows anatomically and functionally specific responses to sequential but not temporal or image changes.

Sequential information permeates our daily lives, such as when listening to music. These sequences are potentially abstract in that they do not depend on the exact identity of the stimuli (pitch of the notes), but on the rule that they follow (interval between them). Previously, we showed that a subregion of monkey lateral prefrontal cortex (LPFC), area 46, responds to abstract visual sequences in a manner that parallels human responses. However, area 46 has several mapped subregions and abstract sequences require of multiple stimulus features (such as stimulus and time), leaving open questions as to the specificity of responses in the LPFC. To determine the anatomical and functional specificity of abstract visual sequence responses within area 46 subregions, we used awake functional magnetic resonance imaging in three male macaque monkeys during two no-report visual tasks. One task presented images in an abstract visual sequence; the other used the same timing properties and image variation, but no sequential information. We found, using subdivisions from a multimodal parcellation of area 46, that responses to abstract visual sequences were unique to the posterior fundus of area 46, which did not respond to changes in timing or image alone. In contrast, posterior shoulder regions of area 46 showed selectivity to more concrete stimulus changes (i.e., timing and image). These results align with organizational hierarchies observed in monkeys and humans, and suggest that interactions between adjacent LPFC subregions is key scaffolding for complex daily behaviors.