A tunable refractive index matching medium for live imaging cells, tissues and model organisms.
Tobias BootheLennart HilbertMichael HeideLea BerningerWieland B HuttnerVasily ZaburdaevNadine L VastenhouwEugene W MyersDavid N DrechselJochen C RinkPublished in: eLife (2017)
In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids.
Keyphrases
- high resolution
- image quality
- cataract surgery
- endothelial cells
- induced apoptosis
- single molecule
- optical coherence tomography
- computed tomography
- induced pluripotent stem cells
- gene expression
- single cell
- subarachnoid hemorrhage
- cell therapy
- magnetic resonance imaging
- gram negative
- oxidative stress
- magnetic resonance
- mesenchymal stem cells
- dual energy
- mass spectrometry
- brain injury