Metabolic signatures of osteoarthritis in urine using liquid chromatography-high resolution tandem mass spectrometry.
Salah AbdelrazigCatharine A OrtoriMichael DohertyAna M ValdesVictoria ChapmanDavid A BarrettPublished in: Metabolomics : Official journal of the Metabolomic Society (2021)
Urinary metabolic profiles were altered in inflammatory knee OA subjects compared to those with non-inflammatory OA and non-OA controls. These altered profiles associated with perturbed activity of the TCA cycle, pyruvate and amino acid metabolism linked to inflammation, oxidative stress and collagen destruction. Of note, 2-keto-glutaramic acid level was > eightfold higher in the inflammatory OA patients compared to non-OA control, signalling a possible perturbation in glutamine metabolism related to OA progression.
Keyphrases
- knee osteoarthritis
- oxidative stress
- tandem mass spectrometry
- liquid chromatography
- ultra high performance liquid chromatography
- high resolution
- mass spectrometry
- high performance liquid chromatography
- simultaneous determination
- gas chromatography
- high resolution mass spectrometry
- amino acid
- end stage renal disease
- dna damage
- ejection fraction
- newly diagnosed
- rheumatoid arthritis
- total knee arthroplasty
- induced apoptosis
- diabetic rats
- prognostic factors
- peritoneal dialysis
- patient reported outcomes
- ms ms