A cell suspension based uptake method to study high affinity glucosinolate transporters.

Deepti M NambiarJuhi KumariGulab C AryaAmarjeet K SinghNaveen Chandra Bisht

Published in: Plant methods (2020)

The transporter assay system described in this study holds potential for studying sub-functionalization amongst GTR homologs present across Brassicaceae family. The fast growing CCL-1 cells, confer the benefits of an in vitro system for quick assays and is plant based thus enabling optimal expression without sequence modifications. The efficient functioning of the GTR transporters in the heterologous CCL-1 opens the possibility of using this plant cell suspension system for functional characterization of other metabolite transporters.

Keyphrases

single cell
high throughput
induced apoptosis
stem cells
liver injury
cell proliferation
cell cycle arrest
binding protein
climate change
drug induced
plant growth
bacillus subtilis