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Transgene stacking in potato using the GAANTRY system.

Kent F McCueEthan GardnerRonald ChanRoger ThilmonyJames G Thomson
Published in: BMC research notes (2019)
The 28.5 kilobase 10-stack T-DNA, was introduced into Lenape potato plants with a 32% transformation efficiency. Molecular and phenotypic characterization confirmed that six of the seven tested independent transgenic lines carried the entire desired construct, demonstrating that the GAANTRY 10-stack strain can be used can be used in a tissue culture-based callus transformation method to efficiently generate transgenic potato plants. Analysis using droplet digital PCR showed that most of the characterized events carry one or two copies of the 10-stack transgenes and that 'backbone' DNA from outside of the T-DNA was absent in the transgenic plants. These results demonstrate that the GAANTRY system efficiently generates high quality transgenic potato plants with a large construct of stacked transgenes.
Keyphrases
  • circulating tumor
  • single molecule
  • cell free
  • nucleic acid
  • high throughput
  • single cell
  • circulating tumor cells