Determination of cold-adapted influenza virus (Orthomyxoviridae: Alphainfluenzavirus ) polymerase activity by the minigenome method with a fluorescent protein.

P A Ivanov A V Lyashko Vladimir Kost Natalia F Lomakina A A Rtishchev N I Bunkova T A Timofeeva M A Balanova S A Ionov D V Gorikov S G Markushin

Published in: Voprosy virusologii (2023)

Polymerase of virus A/Krasnodar/101/35/59 (H2N2) has higher replicative and transcriptional activity at 33 °C than at 37 °C. Its transcriptional activity is more temperature-dependent than its replicative activity. The replicative and transcriptional activity of polymerase A/Puerto Rico/8/34 virus (H1N1, Mount Sinai variant) have no significant differences and do not depend on temperature.

Keyphrases

gene expression
transcription factor
oxidative stress
quantum dots
heat shock
high resolution
fluorescent probe
heat stress
simultaneous determination
solid phase extraction
protein protein