Direct visualization of the conformational change of FUS/TLS upon binding to promoter-associated non-coding RNA.

Nesreen Hamad Hiroki Watanabe Takayuki UchihashiRiki KurokawaTakashi Nagata Masato Katahira

Published in: Chemical communications (Cambridge, England) (2021)

High-speed AFM revealed the conformational change of fused in sarcoma (FUS) from a compact to an extended structure upon binding of non-coding RNA, which is supposed to allow FUS to bind to CBP/p300 for transcriptional interference. Thus, a mechanistic insight into transcription regulation by FUS and non-coding RNA is provided.

Keyphrases

high speed
atomic force microscopy
transcription factor
molecular dynamics simulations
single molecule
molecular dynamics
gene expression
nucleic acid
high resolution
single cell
oxidative stress
binding protein
low cost