A self-cascade system based on Ag nanoparticle/single-walled carbon nanotube nanocomposites as an enzyme mimic for ultrasensitive detection of L-cysteine.

L-Cysteine, widely used in medicine and the food industry, is of great essentiality to organisms and the food quality. Given that current detection approaches require exacting lab conditions and tedious sample treatment, there is a pressing demand for developing a method that possesses advantages of user friendliness, prominent performance, and cost-effectiveness. Herein, a self-cascade system was developed for the fluorescence detection of L-cysteine based on the ingenious performance of Ag nanoparticle/single-walled carbon nanotube nanocomposites (AgNP/SWCNTs) and DNA-templated Ag nanoclusters (DNA-AgNCs). The fluorescence of DNA-AgNCs could be quenched on account of the adsorption of DNA-AgNCs on AgNP/SWCNTs by π-π stacking. With the cooperation of Fe 2+ , AgNP/SWCNTs with oxidase and peroxidase-like activities could catalyze the oxidation of L-cysteine to produce cystine and hydrogen peroxide (H 2 O 2 ) and then break the O-O bond of H 2 O 2 to generate a hydroxyl radical (·OH), which could cleave the DNA strand into different sequence fragments which subsequently peeled off from the AgNP/SWCNTs, resulting in a "turn-on" fluorescence response. In this paper, AgNP/SWCNTs with multi-enzyme activities was synthesized enabling the reaction to proceed in just one step. The successful preliminary applications for the L-cysteine detection in pharmaceutical, juice beverage, and serum samples indicated that the developed method exhibited great potential in medical diagnosis, food monitoring, and the biochemical field, which also broadened the horizon for follow-up research.