The P2×7 receptor is a master regulator of microparticle and mitochondria exchange in mouse microglia.
Simonetta FalzoniValentina Vultaggio-PomaPaola ChiozziMario TarantiniElena AdinolfiPaola BoldriniAnna Lisa GiulianiGiampaolo MorcianoYong TangDariusz C GóreckiFrancesco Di VirgilioPublished in: Function (Oxford, England) (2024)
Microparticles (MPs) are secreted by all cells where they play a key role in intercellular communication, differentiation, inflammation, and cell energy transfer. P2×7 receptor (P2×7R) activation by extracellular ATP (eATP) causes a large MP release and affects their contents in a cell-specific fashion. We investigated MP release and functional impact in microglial cells from P2×7R-WT or P2×7R-KO mice, as well as mouse microglial cell lines characterized for high (N13-P2×7RHigh) or low (N13-P2×7RLow) P2×7R expression. P2×7R stimulation promoted release of a mixed MP population enriched with naked mitochondria. Released mitochondria were taken up and incorporated into the mitochondrial network of the recipient cells in a P2×7R-dependent fashion. NLRP3 and the P2×7R itself were also delivered to the recipient cells. MP transfer increased the energy level of the recipient cells and conferred a pro-inflammatory phenotype. These data show that the P2×7R is a master regulator of intercellular organelle and MP trafficking in immune cells.
Keyphrases
- induced apoptosis
- cell cycle arrest
- cell death
- inflammatory response
- single cell
- type diabetes
- signaling pathway
- cell therapy
- machine learning
- lipopolysaccharide induced
- reactive oxygen species
- stem cells
- quantum dots
- metabolic syndrome
- adipose tissue
- energy transfer
- cell proliferation
- pi k akt
- artificial intelligence
- bone marrow
- mesenchymal stem cells
- mass spectrometry
- spinal cord injury
- atomic force microscopy