Extrachromosomal DNA-mediated glyphosate resistance in Italian ryegrass.

We report the extrachromomal DNA-mediated glyphosate resistance in Italian ryegrass. Physical mapping of amplified copies of EPSPS gene in Italian ryegrass by FISH gives us a clue that the amplified copies of EPSPS gene may be present in the extrachromosomal DNA elements. Further analysis by PFGE followed by DNA blotting revealed that the extrachromosomal DNA containing EPSPS is of ~400 kb similar in size with that of eccDNA replicon in Amaranthus palmeri. This article is protected by copyright. All rights reserved.