Multiplexed mass spectrometry of individual ions improves measurement of proteoforms and their complexes.
Jared O KafaderRafael D MelaniKenneth R DurbinBon IkwuagwuBryan P EarlyRyan T FellersSteven C BeuVlad ZabrouskovAlexander A MakarovJoshua T MazeDeven L ShinholtPing F YipDanielle Tullman-ErcekMichael W SenkoPhilip D ComptonMichael P SnyderPublished in: Nature methods (2020)
An Orbitrap-based ion analysis procedure determines the direct charge for numerous individual protein ions to generate true mass spectra. This individual ion mass spectrometry (I2MS) method for charge detection enables the characterization of highly complicated mixtures of proteoforms and their complexes in both denatured and native modes of operation, revealing information not obtainable by typical measurements of ensembles of ions.
Keyphrases
- mass spectrometry
- liquid chromatography
- quantum dots
- gas chromatography
- high resolution
- high performance liquid chromatography
- capillary electrophoresis
- aqueous solution
- high resolution mass spectrometry
- water soluble
- tandem mass spectrometry
- multiple sclerosis
- ionic liquid
- ultra high performance liquid chromatography
- health information
- protein protein
- social media
- real time pcr
- simultaneous determination