Novel Enhancer for Luminol-AuNP Electrochemiluminescence and Decoration on RNA Membranes for Effective Cytosensing.

Herein, a simple and nice method to synthesize RNA membrane nanostructures for a controlled capture and cytosensing by aptamer and luminol-AuNPs (luAu NPs) is presented. The RNA membrane made up of many RNA transcripts was prepared by a one-pot reaction, the rolling circle transcription (RCT) process using rNTP and RNA polymerase, so the efficient RNA membrane could be obtained successfully at a much lower cost than the system used for RNA molecule synthesis commercially. The modifications of the NH 2 group could not degrade the stability of RNA membrane in the serum. The prepared RNA membrane nanostructures showed interesting capture efficiencies toward the Ramos cell due to the effect of aptamer-ligand interactions on the nanostructures. Additionally, based on the detection of the luAu NP electrochemiluminescence (ECL) system, a novel enhancing method was employed by 3-aminopropyl-triethoxysilane (APS) for luAu NP ECL, which has never been reported, to improve the sensitivity. The ECL intensity was proportional to the target cell amounts, and the dynamic range was 50-5000 cells. The detection limit of the target cell amounts reached level 50. The specificity test and recovery test showed that the method had a good selectivity, stability, and repeatability. Therefore, this method will provide a favorable analysis for biosensing.