The protective effect of biomineralized BSA-Mn 3 O 4 nanoparticles on HUVECs investigated by atomic force microscopy.
Xinyue GuoWeiqi ZhouYanxue GuanJuan QinBailin ZhangMiaomiao ZhangJilin TangPublished in: The Analyst (2022)
Manganese-based nanozymes have been widely used in the field of cell protection due to their various enzyme-mimicking activities, but their effect on the mechanical properties of cells is not yet known. Here, bovine serum albumin-modified Mn 3 O 4 nanoparticles (BSA-Mn 3 O 4 NPs) with good biocompatibility were synthesized by a one-step biomineralization method using BSA as a template. BSA-Mn 3 O 4 NPs possess scavenging activity against superoxide free radicals (O 2 ˙ - ), hydroxyl radicals (˙OH) and hydrogen peroxide (H 2 O 2 ). The excellent reactive oxygen species (ROS) scavenging activity of BSA-Mn 3 O 4 NPs enables them to effectively reduce the intracellular ROS level, thus mitigating the damage of oxidative stress on human umbilical vein endothelial cells (HUVECs). Subsequently, the intracellular antioxidant mechanism of the BSA-Mn 3 O 4 NPs was further investigated. The results show that the BSA-Mn 3 O 4 NPs could inhibit the depolymerization of F-actin, help cells maintain their normal morphology, and reduce the decrease in Young's modulus of cells caused by oxidative stress.
Keyphrases
- induced apoptosis
- oxidative stress
- reactive oxygen species
- hydrogen peroxide
- atomic force microscopy
- room temperature
- cell cycle arrest
- dna damage
- transition metal
- endoplasmic reticulum stress
- endothelial cells
- cell death
- oxide nanoparticles
- metal organic framework
- signaling pathway
- ischemia reperfusion injury
- bone marrow
- stem cells
- single molecule
- cell proliferation
- middle aged