Determination of Free Histidine in Complex Hair Care Products with Minimum Sample Preparation Using Cation-Exchange Chromatography and Post Column Derivatization.
Apostolia TsiasiotiConstantinos K K ZacharisParaskevas D TzanavarasPublished in: Molecules (Basel, Switzerland) (2023)
In this communication, we describe the first analytical method for the determination of free histidine in hair care products (shampoos and conditioners). Cation-exchange chromatography combined with postcolumn derivatization and fluorimetric detection enabled the accurate (recovery: 83.5-114.8%) and precise (2.4-5.6% RSD) determination of free histidine without matrix interferences at concentration levels down to 1.5 mg kg -1 . Real commercially available samples were found to contain the amino acid at levels ranging between 70 and 535 mg kg -1 .
Keyphrases
- solid phase extraction
- liquid chromatography
- high performance liquid chromatography
- tandem mass spectrometry
- molecularly imprinted
- mass spectrometry
- liquid chromatography tandem mass spectrometry
- ultra high performance liquid chromatography
- simultaneous determination
- gas chromatography mass spectrometry
- high resolution mass spectrometry
- gas chromatography
- healthcare
- palliative care
- amino acid
- ionic liquid
- quality improvement
- high speed
- ms ms
- pain management
- label free
- health insurance
- affordable care act
- loop mediated isothermal amplification