2-Nitroimidazole based fluorescent probes for nitroreductase; monitoring reductive stress in cellulo.
X AoS A BrightN C TaylorRobert B P ElmesPublished in: Organic & biomolecular chemistry (2017)
Two 2-nitroimidazole-1,8-naphthalimide conjugates, 1 and 2, have been synthesised as fluorescence probes for the detection of reductive stress in HeLa cells. The 4-substituted derivative 1 was shown to act as a highly sensitive and selective substrate for nitroreductase where it exhibited a clear blue to green ratiometric fluorescence response visible to the naked eye. Moreover, biological studies demonstrated 1 could be activated in cellulo where the impact of reductive stress was easily monitored using confocal microscopy and flow cytommetry.
Keyphrases
- living cells
- fluorescent probe
- single molecule
- cell cycle arrest
- quantum dots
- small molecule
- energy transfer
- induced apoptosis
- stress induced
- label free
- fluorescence imaging
- cell proliferation
- oxidative stress
- heat stress
- cancer therapy
- case control
- molecular dynamics simulations
- nitric oxide
- loop mediated isothermal amplification
- photodynamic therapy
- nucleic acid
- simultaneous determination
- amino acid