On-line capillary electrophoresis/laser-induced fluorescence/mass spectrometry analysis of glycans labeled with Teal™ fluorescent dye using an electrokinetic sheath liquid pump-based nanospray ion source.
Shaheer KhanJenkuei LiuZoltan SzaboBaburaj KunnummalXiaorui HanYilan OuyangRobert J LinhardtQiangwei XiaPublished in: Rapid communications in mass spectrometry : RCM (2018)
This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
Keyphrases
- mass spectrometry
- capillary electrophoresis
- liquid chromatography
- gas chromatography
- high performance liquid chromatography
- quantum dots
- energy transfer
- high resolution
- living cells
- ms ms
- ionic liquid
- highly efficient
- tandem mass spectrometry
- cell surface
- multiple sclerosis
- room temperature
- label free
- big data
- pet imaging
- simultaneous determination
- cell free
- fluorescent probe
- positron emission tomography