Proteotranscriptomics assisted gene annotation and spatial proteomics of Bombyx mori BmN4 cell line.

We show that proteotranscriptomics is an efficient, cost-effective and accurate approach to improve previous annotations or generate new gene models. As this technique is based on de-novo transcriptome assembly, it provides the possibility to study any species also in the absence of genome sequence information for which proteogenomics would be impossible.