Noncontact Diffuse Reflectance Spectroscopy of Synovial Fluid Samples for Rapid Identification of Infections.
Erin E DrewkeRobert L BrandCaroline G GeelsHanna K JensenKevin WongJarret D SandersNarasimhan RajaramPublished in: Journal of biophotonics (2024)
Severe joint infections, such as septic arthritis, require rapid diagnostic testing of the synovial fluid aspirated from joints level so that a surgical team can be assembled quickly. We present a diffuse reflectance spectroscopy (DRS) system for noncontact determination of infection. Using a light-tight syringe holder and fiber optic probe, diffusely reflected light from 475 to 655 nm was acquired from 18 patient samples through the wall of a syringe in a noncontact and sterile manner. We determined the reflectance ratios at two different wavelengths-R 490 /R 600 and R 580 /R 600 and found statistically significant differences (p < 0.05) in both ratios between the infected and noninfected groups. Critically, the R 490 /R 600 and R 580 /R 600 ratios were significantly correlated with clinical biomarkers-the white blood cell (WBC) and red blood cell (RBC) counts, respectively. This study demonstrates the potential of DRS as a rapid diagnostic tool for joint infections.
Keyphrases
- red blood cell
- loop mediated isothermal amplification
- high resolution
- single molecule
- low grade
- acute kidney injury
- single cell
- palliative care
- photodynamic therapy
- optical coherence tomography
- blood brain barrier
- case report
- stem cells
- mesenchymal stem cells
- quality improvement
- risk assessment
- peripheral blood
- mass spectrometry
- living cells
- drug induced
- light emitting