Synthesis and validation of ultrasensitive stripping voltammetric sensor based on polypyrrole@ZnO/Fe₃O₄ core-shell nanostructure for picomolar detection of artesunate and dopamine drugs.

A stripping voltammetric sensor for ultrasensitive detection of artesunate (ART) and dopamine HCl (DA) has been successfully developed using a Ppy@ZnO/Fe₃O₄ core-shell nanocomposite ([PZM])-modified carbon paste sensor (MCPS). Fourier transform infrared spectroscopy, X-ray diffraction, field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, dynamic light scattering, Brunauer-Emmett-Teller surface area method, and high-resolution transmission electron microscopy were used to characterize the physicochemical properties of the nanomaterials. Noteworthily, the morphology of [PZM] reveals a spherical core-shell nanostructure with an increase in the average diameter range of 20-37.5 nm (specific surface area (SSA) of 28.5 m² g^-1 (0.0247 cm³ g^-1)) when compared with the average diameter range 7.5-15.7 nm (SSA of 5.43 m² g^-1 (0.0111 cm³ g^-1)) of ZnO/Fe₃O₄[ZM]. The [PZM] MCPS provided the best electroactive surface area (0.078 cm²) and the least electrocatalytic activity (<i>R</i>_st = 370 Ω). Furthermore, the MCPS showed low detection limits (LODs) of 0.092 pg mL^-1 (0.24 pM) and 0.0046 pg mL^-1 (0.03 pM) for ART and DA, respectively. Moreover, LODs were found to be 0.029 pg mL^-1 (0.75 pM) and 0.014 pg mL^-1 (0.09 pM) for ART mixed with 0.7 pM of DA (ART₁) and DA in the presence of 2.0 pM of ART drug (DA₁), respectively. In addition, the MCPS revealed a proper repeatability, reproducibility, and storage stability (93.5-90.48%). During the routine analysis, the [PZM] MCPS detected ART and DA concentrations in human urine, without interference.