Efficient generation of male germ-like cells derived during co-culturing of adipose-derived mesenchymal stem cells with Sertoli cells under retinoic acid and testosterone induction.
Yanxia LuoLili XieAli MohsinWaqas AhmedChenze XuYan PengHaifeng HangYingping ZhuangJu ChuMeijin GuoPublished in: Stem cell research & therapy (2019)
These results suggested that TM4 cells could efficiently stimulate in vitro generation of MGLCs during co-culturing of ADMSCs under RA and T treatment. Conclusively, the ADMSCs co-cultured with TM4 cell under RA and T induction stimulate the efficient generation of MGLCs in vitro through activating TGFβ-SMAD2/3, JAK2-STAT3, and AKT pathways. Among them, JAK2-STAT3 and AKT pathways are being first reported to show involvement of in vitro generation of MGLCs during ADMSC co-culturing with SCs.
Keyphrases
- induced apoptosis
- signaling pathway
- cell cycle arrest
- rheumatoid arthritis
- cell proliferation
- transforming growth factor
- epithelial mesenchymal transition
- oxidative stress
- cell therapy
- stem cells
- endoplasmic reticulum stress
- disease activity
- cell death
- insulin resistance
- endothelial cells
- pi k akt
- mesenchymal stem cells
- systemic sclerosis
- smoking cessation