Targeted gold nanoshelled hybrid nanocapsules encapsulating doxorubicin for bimodal imaging and near-infrared triggered synergistic therapy of Her2-positve breast cancer.
Qi DongCaifeng WanHong YangDongdong ZhengLi XuZhiguo ZhouShaowei XieJing DuFenghua LiPublished in: Journal of biomaterials applications (2020)
A multifunctional targeted nanoplatform combining photothermal therapy and chemotherapy has emerged as a promising strategy for comprehensive therapies of breast cancer. In this study, we constructed human epidermal growth factor receptor 2 (Her2)-targeted gold nanoshelled poly(lactic-co-glycolic acid) hybrid nanocapsules encapsulating perfluorooctyl bromide, superparamagnetic iron oxide nanoparticles, and doxorubicin (Her2-GPDH nanocapsules) as theranostic agent for bimodal ultrasound/magnetic resonance imaging and synergistic photothermal-chemotherapy of Her2-postive breast cancer cells. Her2-GPDH nanocomposites possessed well-defined spherical morphology, and the average diameter was about 296 nm with good dispersion. Targeting assays demonstrated that Her2-GPDH nanocapsules exhibited higher targeting binding to Her2-positive SKBR3 cells than Her2-negative MDA-MB-231cells. The encapsulation efficiency and the loading content of doxorubicin in Her2-GPDH nanocapsules were 39 ± 1.45% and 3.8 ± 0.52%, respectively, and the agent exhibited pH-responsive and near-infrared light-triggered stepwise release behavior of doxorubicin. In vitro, the agent had potential to serve as feasible candidate for ultrasound imaging and T2-weighted magnetic resonance imaging with a relatively high relaxivity. Cell experiments confirmed that the agent had significant photothermal cytotoxicity on SKBR3 cells, and the combined photothermal-chemotherapy could significantly enhance the anti-tumor effect. In summary, the present Her2-GPDH nanocapsules, a novel multifunctional nanoplatform, will offer a new way for early bimodal molecular-level diagnosis and synergistic treatment of Her2-positve breast cancer.
Keyphrases
- cancer therapy
- drug delivery
- magnetic resonance imaging
- induced apoptosis
- cell cycle arrest
- epidermal growth factor receptor
- breast cancer cells
- photodynamic therapy
- cell death
- locally advanced
- stem cells
- contrast enhanced
- high resolution
- squamous cell carcinoma
- cell proliferation
- single cell
- oxidative stress
- pi k akt
- bone marrow
- mass spectrometry
- wastewater treatment
- fluorescence imaging
- metal organic framework
- rectal cancer
- smoking cessation
- network analysis
- diffusion weighted imaging
- human health
- chemotherapy induced
- replacement therapy