Fluorescence Quenching-Based Mechanism for Determination of Hypochlorite by Coumarin-Derived Sensors.
Karolina StarzakArkadiusz P MatwijczukBernadette CreavenAlicja MatwijczukSlawomir WybraniecDariusz KarczPublished in: International journal of molecular sciences (2019)
A fluorescence quenching-based mechanism for the determination of hypochlorite was proposed based on spectroscopic and chromatographic studies on the hypochlorite-sensing potency of three structurally similar and highly fluorescent coumarins. The mode of action was found to rely upon a chlorination of the coumarin-based probes resulting from their reaction with sodium hypochlorite. Importantly, the formation of chlorinated derivatives was accompanied by a linear decrease in the fluorescence intensities of the probes tested. The results obtained suggest the applicability of a coumarin-dependent hypochlorite recognition mechanism for the detection of, as well as for quantitative determination of, hypochlorite species in vitro.
Keyphrases
- single molecule
- energy transfer
- living cells
- fluorescent probe
- solid phase extraction
- molecularly imprinted
- small molecule
- fluorescence imaging
- quantum dots
- drinking water
- label free
- mass spectrometry
- simultaneous determination
- nucleic acid
- loop mediated isothermal amplification
- photodynamic therapy
- real time pcr
- sensitive detection
- structure activity relationship