Screening populations for copy number variation using genotyping-by-sequencing: a proof of concept using soybean fast neutron mutants.
Marc-André LemayDavoud TorkamanehGuillem RigaillBrian BoyleAdrian O StecRobert M StuparFrançois BelzilePublished in: BMC genomics (2019)
This study provides a proof of concept that GBS can be used as an affordable high-throughput method for assessing CNVs in fast neutron mutants. The modularity of this GBS approach allows combining as many different libraries or sequencing runs as is necessary for reaching the goals of a particular study. This method should enable the low-cost genome-wide characterization of hundreds to thousands of individuals in fast neutron mutant populations or any population with large genomic deletions and duplications.