Preparation of Photocatalytic and Antibacterial MOF Nanozyme Used for Infected Diabetic Wound Healing.
Xingjun ZhaoLinna ChangYanan HuShibo XuZepeng LiangXiuli RenXifan MeiZhenhua ChenPublished in: ACS applied materials & interfaces (2022)
Bacterial infection has been a considerable obstacle for diabetic wound healing. A multifunctional nanoplatform used as nanozyme for bacterial infected diabetic wound is extremely attractive. Therefore, gold nanoclusters modified zirconium-based porphyrin metal-organic frameworks (Au NCs@PCN) were constructed by an in situ growth method. Through SEM, TEM, and EDS mapping, the surface of ellipsoid-shaped particles around 190 nm was observed to be evenly interspersed with 5-8 nm gold nanoclusters. Notably, Au NCs@PCN exhibits excellent performance in exciting ROS generation and photothermal effects. Under near-infrared (NIR) laser irradiation, Au NCs@PCN can be heated to 56.2 °C and produce ROS, showing an effective killing effect on bacteria. Antibacterial studies showed that Au NCs@PCN inhibited MRSA and Amp r E. coli by destroying membrane structure and inducing protein leakage up to 95.3% and 90.6%, respectively. Animal experiments showed that Au NCs@PCN treated diabetic rats had reduced wound coverage to 2.7% within 21 days. The immunoblot analysis showed that proangiogenic and proepithelial cell proliferation factors were expressed significantly up-regulated. These results prove that Au NCs@PCN with photocatalytic and nanozyme activity has a broad application prospect for promoting diabetic infected wound healing.
Keyphrases
- wound healing
- sensitive detection
- reduced graphene oxide
- photodynamic therapy
- metal organic framework
- visible light
- cell proliferation
- quantum dots
- diabetic rats
- gold nanoparticles
- cancer therapy
- drug delivery
- oxidative stress
- cell death
- dna damage
- escherichia coli
- high resolution
- staphylococcus aureus
- fluorescence imaging
- small molecule
- transcription factor
- reactive oxygen species
- cell cycle
- fluorescent probe
- energy transfer
- protein kinase
- amino acid
- label free
- health insurance
- protein protein
- high density
- radiation therapy
- affordable care act