A snapshot of 100 years of discovery.

With a century of literature behind Journal of Experimental Biology (JEB) in 2023, I look at some of the extraordinary papers contained within its archive. From publishing Nobel Prize-inspiring discoveries to founding fields and solving long-standing mysteries, the journal has been at the hub of experimental biology for 10 decades, leading the way and shining a light on the physiology of many remarkable animal species. In this Perspective, I highlight some of the key players in the field, summarise their seminal works and consider their long-term impact as JEB embarks on its next 100 years.