Metabolome and Transcriptome Analysis of Sulfur-Induced Kiwifruit Stem Laccase Gene Involved in Syringyl Lignin Synthesis against Bacterial Canker.
Zhuzhu ZhangYouhua LongXianhui YinWeizhen WangWenzhi LiTingting ChenJia ChenXuetang ChenBince WangJiling MaPublished in: Journal of agricultural and food chemistry (2023)
Kiwifruit canker is caused by Pseudomonas syringae pv . actinidiae and is one of the most destructive diseases of kiwifruit worldwide. Sulfur can improve the deposit of lignin in kiwifruit stems and induce disease resistance, but the action mechanism at the molecular level remains unclear. This omics-based study revealed that sulfur-induced S lignin synthesis contributes to disease resistance. Histological staining verified sulfur-enhanced total lignin deposition in kiwifruit stems. High-performance liquid chromatography and confocal Raman microscopy showed that sulfur-activated S lignin was mainly deposited in the cell corner. Metabolome and transcriptome analysis revealed that the levels of phenylpropanoid pathway S lignin precursors sinapic acid and sinapyl alcohol were significantly increased and 16 laccase genes were upregulated. Sulfur-induced resistance defense promoted elevated laccase activity by activating the laccase genes, participating in sinapic acid and sinapyl alcohol substance synthesis, and ultimately polymerizing S lignin at cell corner against kiwifruit canker disease.
Keyphrases
- ionic liquid
- single cell
- high glucose
- high performance liquid chromatography
- diabetic rats
- genome wide
- cell therapy
- escherichia coli
- mass spectrometry
- drug induced
- high resolution
- genome wide identification
- simultaneous determination
- oxidative stress
- endothelial cells
- alcohol consumption
- ms ms
- high speed
- pseudomonas aeruginosa
- raman spectroscopy
- flow cytometry
- bioinformatics analysis