A sequentially activated bioluminescent probe for observation of cellular H2O2 production induced by cysteine.

Kaiqing MaYongkang YueLingling ZhaoJian Bin Chao Caixia Yin

Published in: Chemical communications (Cambridge, England) (2021)

We report herein a caged luciferin probe Cy-Hy as a sequentially activated probe to selectively and sensitively sense L-Cys and H2O2. The probe displayed fluorescence and bioluminescence responses toward the two analytes. Utilizing the present probe, cellular excess L-Cys-induced H2O2 up-regulation was observed for the first time in living MDA-MB-231 cells.

Keyphrases

living cells
quantum dots
fluorescent probe
single molecule
cell cycle arrest
induced apoptosis
energy transfer
oxidative stress
endothelial cells
breast cancer cells
drug induced