Response to Comments on "Monoclonal Antibody Discovery Based on Precise Selection of Single Transgenic Hybridomas with an On-Cell-Surface and Antigen-Specific Anchor".

Listek et al. described some technical concerns for the single transgenic hybridoma selection method (STHSM) that we developed. In this work, we developed the STHSM to screen hapten-specific hybridoma cells based on a streptavidin-labeled antigen originally proposed by Listek et al. that was used for the selection of OVA-specific hybridoma. Due to the obvious differences between hapten with a single epitope and protein with multiple epitopes, we significantly innovated the specific procedures in the STHSM that could answer the questions raised by Listek's group.