Bacillus thuringiensis subsp. israelensis producing endochitinase ChiA74Δsp inclusions and its improved activity against Aedes aegypti.
E O Juárez-HernándezL E Casados-VázquezM C del Rincón-CastroR Salcedo-HernándezD K BideshiJosé E Barboza-CoronaPublished in: Journal of applied microbiology (2016)
We report for the first time the engineering of Bti to produce spore-parasporal body-ChiA74∆sp inclusions in the same sporangium, which are released together following autolysis. Our work lays a foundation for engineering Bti to produce more efficacious combinations of Cry4Aa, Cry4Ba, Cry11Aa, Cyt1Aa and chitinase inclusions.