Multimodal Characterization of Cardiac Organoids Using Integrations of Pressure-Sensitive Transistor Arrays with Three-Dimensional Liquid Metal Electrodes.
Moohyun KimJae Chul HwangSungjin MinYoung-Geun ParkSuran KimEnji KimHunkyu SeoWon Gi ChungJakyoung LeeSeung-Woo ChoJang-Ung ParkPublished in: Nano letters (2022)
Herein, we present an unconventional method for multimodal characterization of three-dimensional cardiac organoids. This method can monitor and control the mechanophysiological parameters of organoids within a single device. In this method, local pressure distributions of human-induced pluripotent stem-cell-derived cardiac organoids are visualized spatiotemporally by an active-matrix array of pressure-sensitive transistors. This array is integrated with three-dimensional electrodes formed by the high-resolution printing of liquid metal. These liquid-metal electrodes are inserted inside an organoid to form the intraorganoid interface for simultaneous electrophysiological recording and stimulation. The low mechanical modulus and low impedance of the liquid-metal electrodes are compatible with organoids' soft biological tissue, which enables stable electric pacing at low thresholds. In contrast to conventional electrophysiological methods, this measurement of a cardiac organoid's beating pressures enabled simultaneous treatment of electrical therapeutics using a single device without any interference between the pressure signals and electrical pulses from pacing electrodes, even in wet organoid conditions.
Keyphrases
- high resolution
- induced pluripotent stem cells
- left ventricular
- reduced graphene oxide
- ionic liquid
- carbon nanotubes
- solid state
- endothelial cells
- pain management
- cardiac resynchronization therapy
- magnetic resonance
- heart failure
- gold nanoparticles
- small molecule
- atrial fibrillation
- contrast enhanced
- diabetic rats
- replacement therapy
- high speed