Anticancer and Anti-Neuroinflammatory Constituents Isolated from the Roots of Wasabia japonica .
Jong Eel ParkTae Hyun LeeSong Lim HamLalita SubediSeong Min HongSun Yeou KimSang Un ChoiChung Sub KimKang Ro LeePublished in: Antioxidants (Basel, Switzerland) (2022)
Wasabi ( Wasabia japonica (Miq.) Matsum.) is a pungent spice commonly consumed with sushi and sashimi. From the roots of this plant, a new 2-butenolide derivative ( 1 ) and 17 previously reported compounds ( 2 - 18 ) were isolated and structurally characterized. Their chemical structures were characterized based on the conventional NMR ( 1 H and 13 C, COSY, HSQC, and HMBC) and HRESIMS data analysis. All of these phytochemicals ( 1 - 18 ) were evaluated for their antiproliferative effects on the four human tumor cell lines (A549, SK-OV-3, SK-MEL-2, and MKN-1), for their inhibitory activity on nitric oxide (NO) production in lipopolysaccharide (LPS)-activated BV-2 microglia cells, and for their nerve growth factor (NGF)-releasing effect from C6 glioma cells. Among the isolated compounds, compound 15 showed powerful antiproliferative activities against A549 and SK-MEL-2 cell lines with IC 50 values of 2.10 and 9.08 μM, respectively. Moreover, the new compound 1 exhibited moderate NO inhibition activity with IC 50 value of 45.3 μM.
Keyphrases
- growth factor
- data analysis
- inflammatory response
- nitric oxide
- lps induced
- lipopolysaccharide induced
- induced apoptosis
- high resolution
- endothelial cells
- cell cycle arrest
- toll like receptor
- magnetic resonance
- high intensity
- hydrogen peroxide
- oxidative stress
- cell death
- spinal cord injury
- anti inflammatory
- cell proliferation
- mass spectrometry
- pi k akt
- water soluble
- cell wall