Probe-Free Direct Identification of Type I and Type II Photosensitized Oxidation Using Field-Induced Droplet Ionization Mass Spectrometry.
Chaonan MuWei WangJie WangChu GongDongmei ZhangXinxing ZhangPublished in: Angewandte Chemie (International ed. in English) (2020)
While Type I and Type II photosensitizers are often carefully tailored to achieve their respective advantages in treating different cancers, the identifications of the Type I and II mechanisms as such, the key reaction intermediates, and the consequent oxidation products of the substrates have never been easy. Using our unique home-built field-induced droplet ionization mass spectrometry (FIDI-MS) method that selectively samples molecules at the air-water interface, here we show the facile determination of both Type I and II mechanisms of a poster-child photosensitizer, temoporfin, without the addition of any probes. The unstable doublet radical resulting from the hydrogen abstraction by the triplet temoporfin through the Type I mechanism is captured, manifesting the in situ advantage of FIDI-MS. We anticipate that the method developed in this study can be widely utilized in the future designs of novel photosensitizers and the screening of their photosensitization mechanisms.
Keyphrases
- mass spectrometry
- photodynamic therapy
- gas chromatography
- liquid chromatography
- high glucose
- diabetic rats
- high performance liquid chromatography
- multiple sclerosis
- capillary electrophoresis
- high resolution
- ms ms
- fluorescence imaging
- high throughput
- single cell
- quantum dots
- living cells
- hydrogen peroxide
- drug induced
- healthcare
- small molecule
- endothelial cells
- tandem mass spectrometry
- solid phase extraction
- visible light
- current status
- nitric oxide
- molecularly imprinted
- electron transfer
- single molecule