Au/Pt@ZIF-90 Nanoenzyme Capsule-Based "Explosive" Signal Amplifier for "All-in-Tube" POCT.

The sensitive, convenient, and visual detection of low-concentration disease markers in biological samples has always been a priority in disease diagnosis. However, existing research has been problematic due to complex operation and unsatisfactory sensitivity. Consequently, an "explosive" signal amplification platform based on Au/Pt@ZIF-90 was developed for sensitive visual detection of disease markers. In this study, a controllable and explosively released Au/Pt nanoparticles (NPs) "nanoenzyme capsule" was prepared by encapsulating Au/Pt NPs with excellent peroxidase activity in ZIF-90. This was achieved by adjusting the particle size of ZIF-90 and the encapsulation amount of Au/Pt NPs. Using the prepared capsules as the signal output module and aptamer as the target recognition module, an "All-in-Tube" portable point-of-care (POC) platform was constructed by integrating the Au/Pt@ZIF-90/filter paper and TMB/strips into an Eppendorf (EP) tube. By utilizing specific competitive binding of targets to aptamers, the platform enabled the sensitive and convenient measurement of small molecular disease markers. Taking adenosine as the proof of concept, the portable detection achieved excellent sensitivity. Moreover, the platform can achieve universal detection of various targets by varying the aptamer sequence. This signal amplification strategy provides a design pattern for the detection of low-concentration targets in biological samples and holds significant potential in the fields of disease diagnosis and environmental monitoring.